Police have said Andrew Mukasa of Bajjo Events and a supporter of People Power movement faces treason charges.
Mukasa was arrested on Saturday by security officers and taken into custody.
However, the police spokesperson, Fred Enanga said the Special Investigations Division of the police is investigating Bajjo over a video in which he was recorded threatening to overthrow President Museveni before the next election in 2021.
"The investigation will lead to charges of treason and threatening violence slapped against Mukasa,"Enanga told a news conference on Monday.
In the video that was shared on social media platforms, Bajjo is seen threatening to oust President Museveni's government before 2021 and warning the army against standing in their way during the oust.
Enanga explained that being a lawfully elected President of Uganda,Museveni enjoys all privileges, adding that it is an offence for anyone to threaten ousting him using force.
"We warn the public against spreading the video," he said.
He said investigations are ongoing but noted if complete, the file will be sent to the Director of Public Prosecutions for perusal and resultant arraignment in courts of law.